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Fig. 3. BT ameliorates the inflammatory response by blocking P2X7r-NLRP3 activation in AML-12 or RAW 264.7 cells stimulated with EtOH or LPS. (A) Representative Western blotting analysis for expressions of P2X7r and NLRP3 in AML-12 cells. (B) Immunofluorescence staining of P2X7r expression in AML-12 cells (100× or 600×). (C) Immunofluorescence staining of NLRP3 expression in AML-12 cells (100× or 600×). (D) Representative Western blotting analysis for expressions of P2X7r and NLRP3 in RAW 264.7 cells. (E) Immunofluorescence staining of P2X7r expression in RAW 264.7 cells (600×). (F) Immunofluorescence staining of NLRP3 expression in RAW 264.7 cells (600×). The GAPDH was used as internal reference to normalize the data. For immunofluorescence staining, ##p<0.01 vs normal group; *p<0.05 vs EtOH group or LPS group. ###p<0.001 vs normal group, **p<0.01, ***p<0.001 vs EtOH group, aaap<0.001 vs LPS group, ns, not significant. All histograms represent the mean ± SD of at least three independent assays.
Biomolecules & Therapeutics 2022;30:246~256 https://doi.org/10.4062/biomolther.2021.136
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