Fig. 1. BT regulates lipid deposition in EtOH-stimulated AML-12 cells. (A) Chemical structure of BT. (B) Cell viability of EtOH on AML-12 cells. (C) Cell viability of BT on AML-12 cells. (D) Representative Western blotting analysis for expressions of SREBP1, lipin1 and lipin2. (E) Oil Red O staining present in 400× magnification. (F) Immunofluorescence staining of lipin1 expression in AML-12 cells (100× and 600×). (G) Immunofluorescence staining of lipin2 expression in AML-12 cells (100× and 600×). The GAPDH was used as internal reference to normalize the data. ###p<0.001, ##p<0.01 vs normal group, *p<0.05, **p<0.01, ***p<0.001 vs EtOH group, ns, not significant. All histograms represent the mean ± SD of at least three independent assays.
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