Fig. 5. Effect of inRas37 and BEZ-235 co-treatment on cancer cell migration and invasion. (A) MIA PaCa-2 and PANC-1 cells were scratched and treated with inRas37 (1 μM) and/or BEZ-235 (0.03 μM) for 72 h. The number of migrated cells was measured using the ImageJ software. (B) MIA PaCa-2 and PANC-1 cells were seeded into upper Transwell chambers and treated with inRas37 (1 μM) and/or BEZ-235 (0.03 μM) for 72 h in serum-free media; lower chambers contained completed media. Invading cells were counted using the ImageJ software. Data are presented the means ± SD (*p< 0.05, **p<0.01, and ***p<0.001).
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