Fig. 2. Therapeutic efficacy of inRas37 on cell viability and KRAS downstream signaling. (A) 3D-cultured anchorage-independent cell viability was measured using an MTS assay in MIA PaCa-2 and PANC-1 cells treated with inRas37 (2 or 5 µM) every 2 days for 1 week. (B) Both pancreatic cancer cells (MIA PaCa-2 and PANC-1) were treated with inRas37 (2 or 5 μM) for 6 h after serum starvation for 2 h, and then stimulated with EGF (10 ng/mL) for 10 min. The expressions of p-AKT, p-P70 S6K, p-MEK, and p-ERK levels were determined using western blotting. Data are presented as the means ± SD (*p<0.05, **p<0.01, and ***p<0.001).
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