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Fig. 4. Knockdown of CHOP attenuates shikonin-induced apoptosis in SNU-C5/5-FUR cells. After confirming the successful transfection of siControl and siCHOP cells via Western blot analysis, the inoculated cells were treated with or without shikonin for 48 h. (A) Cells with apoptotic sub-G1 DNA content were detected using flow cytometry following PI staining, and (B) DNA fragmentation was quantitated using ELISA. After 48 h incubation, (C) the apoptotic bodies were identified via Hoechst 33342 nuclear stain and (D) cell viability was evaluated via MTT analysis. (A-D) *Significantly different from siControl-transfected cells (p<0.05); #significantly different from shikonin-treated, siControl-transfected cells (p<0.05).
Biomolecules & Therapeutics 2022;30:265~273 https://doi.org/10.4062/biomolther.2021.118
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