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Fig. 2. Shikonin triggers accumulation of mitochondrial Ca2+ in SNU-C5/5-FUR cells. After cells were loaded with the fluorescent probe, Rhod-2 AM, mitochondrial Ca2+ levels were measured via (A) flow cytometry and (B) confocal microscopy. *Significantly different from control cells (p<0.05). (C) Cells were inoculated in culture medium with or without EGTA (500 mM), and after 16 h, 3.3 µM shikonin was added. After 48 h incubation, the presence of apoptotic bodies was determined using the Hoechst 33342 nuclear staining method. *Significantly different from control cells (p<0.05); #significantly different from shikonin-treated cells (p<0.05).
Biomolecules & Therapeutics 2022;30:265~273 https://doi.org/10.4062/biomolther.2021.118
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