Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 6. The angiostatic activity of NOEY2-N and eight mutants of NOEY2-N. (A) After pre-treatment with/without VEGF, transfection with control, NOEY2-N, K15A, K15D, K15N, K15S, R16A, R16D, R16N, or R16S, respectively. Capillary-like tubule structures were imaged with a digital camera attached to an inverted microscope. Scale bars indicate 50 µm. (B) Quantitative evaluation of the angiostatic activity. Data presented as the mean length of tube formation in each sample. VEGF-treated expression vector only was used as a positive control. *p<0.05 versus control. Experiments were repeated three times with similar results. (C) SKOV-3 cells were incubated with/without 10 ng/mL VEGF stimulator and were then transfected with control, NOEY2-N or various mutants, respectively. Expression levels of VEGF and HIF-1α affect the proteins associated with angiogenesis control and therefore play vital roles in regulating tumour angiogenesis. Expression levels of VEGF and HIF-1α were visualised using western blotting. Experiments were repeated at least three times with similar results.
Biomolecules & Therapeutics 2021;29:506~518 https://doi.org/10.4062/biomolther.2021.121
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