Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

Download original image
Fig. 5. NOEY2-N and eight mutants induce apoptosis via a caspase-3-dependent pathway. (A) The growth-inhibitory effect of NOEY2-N and eight mutants on endothelial cell proliferation. Cells were maintained and incubated for 3 days with/without VEGF. Counts per minute of [3H] thymidine were calculated using a liquid scintillation counter. Each data point represents triplicate samples, and bars indicate mean ± SD. *p<0.05; **p<0.01 compared to control. (B) Cell proliferation was estimated using the CellTiter-Glo assay system. SKOV-3 cells were maintained at a density of 4.3×103 per well in 96-well plates. After 24 h, cells were transfected with NOEY2-N or various mutants. Data are presented as mean ± SD from three independent experiments. *p<0.05; **p<0.01 versus control. (C) Caspase-3 activity was estimated using a Spectramax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) in fluorescence mode 400 nm (excitation) and 505 nm (emission) according to the manufacturer’s instructions. Enzyme activity was evaluated from fluorescence values according to the formula provided by the manufacturer. Each data point represents triplicate samples, and bars indicate mean ± SD. *p<0.05; **p<0.01 compared to control.
Biomolecules & Therapeutics 2021;29:506~518 https://doi.org/10.4062/biomolther.2021.121
© Biomolecules & Therapeutics