Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 4. The lithocholic acid (LCA)-induced response is specifically mediated by Mrgprb2. (A) LCA-induced increase in intracellular calcium (n=2,350 cells) was suppressed by pretreatment with 10 µM QWF (1,470 cells) in Mrgprb2-expressing HEK293T cells (Mrgprb2-HEK293T). (B) A comparison of relative responses between LCA vs. QWF pretreatment. ***p<0.001. (C) Representative fluorescent images of Mrgprb2-HEK293T at initial (0 s) and peak time points for QWF pretreatment before LCA application. Scale bars=100 µm. (D) LCA did not evoke a noticeable increase in intracellular calcium levels in calcium-free media (n=3,393 cells). (E-G) Responses from various inhibitors on LCA treatment in Mrgprb2-HEK293T. LCA-induced responses were significantly suppressed by SKF96365 (E; a non-specific TRPC blocker, n=2,276 cells), U73122 (F; an inhibitor of PLC, n=1,078 cells), and YM254890 (G; a Gαq inhibitor, n=1,314 cells). (H) A summary of relative responses of pretreatment effects with various inhibitors in the Mrgprb2-HEK293T. ***p<0.001.
Biomolecules & Therapeutics 2022;30:38~47
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