Fig. 2. Both compounds 110 and 187 are selective agonists for GPR43. (A) cAMP levels of GPR41 and GPR43-expressing cells treated with the indicated reagents. Acetate and propionate (10 mM) were added. AR420626, PAAT, compound 110, and compound 187 (10 μM) were added. Data are presented as mean values ± SEM from triplicate experiments. p values <0.05 were significant (**0.005<p<0.01, ***p<0.005). Measurement of (B) cAMP level, (C) Ca2+ flux, and (D) Nano Live Glo luminescence to evaluate the extent of interactions with GPR43 and β-arrestin 2 according to the concentrations of the indicated reagents.
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