Fig. 3. Involvement of caspases in luteolin-induced cell death in human endometriotic cells. (A) 12Z cells were treated with luteolin (15, 30, and 60 μM) for 48 h. Pro-caspase-3, -8, and -9 levels were determined by Western blot assay. β-Actin was used as a control. *p<0.05 as compared the control group. (B) 12Z cells were pretreated with broad caspase inhibitor z-VAD-FMK (50 μM), caspase-3 inhibitor z-DEVD-FMK (50 μM), caspase-8 inhibitor z-IETD FMK (50 μM), and caspase-9 inhibitor z-LEHD-FMK (50 μM) for 30 min, and then treated with luteolin (30 μM) for 48 h. MTT assay wasperformed to determine the cell viability after luteolin treatment. #p<0.05 as compared with the control group, and *p<0.05 as compared with the luteolin-treated group.
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