Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 2. Effects of transduced Tat-Trx1 protein on cell viability in response to oxidative stress. (A) Effect of transduced Tat-Trx1 protein on cell viability. HT-22 cells were pretreated with Tat-Trx1 protein (1-5 μM) for 1 h and exposed to H2O2 (700 μM) for 1 h 30 min. Cell viabilities were estimated using a colorimetric assay using WST-1. Effects of Tat-Trx1 protein on H2O2-induced ROS production and DNA damage. Treatment with Tat-Trx1 protein (5 μM) and Trx1 protein was followed by 1 h treatment with H2O2 (700 μM). Intracellular ROS levels were measured by (B) DCF-DA staining and (C) DNA fragmentation was detected by TUNEL staining. The fluorescence intensity was measured by ELISA plate reader; scale bar=50 μm. The bars in the figure represent the mean ± SEM obtained from 3 independent experiments. *p<0.05 compared with H2O2-treated cells.
Biomolecules & Therapeutics 2021;29:321~330
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