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Fig. 1. Differential production of SARS-CoV-2 in Vero and Calu-3 cells. Vero and Calu-3 cells (5×104 cells/well in 12-well plates) were cultured in DMEM containing 10% FBS. After overnight culture, cells were washed with PBS and SARS-CoV-2 in PBS at a MOI of 0.1 was added into each well (n=3). After 1 h of incubation, the medium was replenished with DMEM containing 2% FBS. (A) Supernatants of virus-infected cell cultures were collected at the indicated times after virus infection to isolate viral RNAs. Virus replication was quantified by real-time RT-PCR analysis of the SARS-CoV-2 RdRP gene. Copy numbers of the RdRP gene in 1 µL of the cDNA samples were calculated using a standard curve of RdRP cDNA. (B) Supernatants of virus-infected Vero and Calu-3 cells (12-well plates) were collected at the indicated times after virus infection. Virus production was quantified by plaque formation assay. *p<0.05, **p<0.01, ***p<0.001.
Biomolecules & Therapeutics 2021;29:273~281 https://doi.org/10.4062/biomolther.2020.226
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