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Fig. 4. Knock-down of Nrf2 attenuates the resorcinol-induced activation of antioxidant pathways. (A) HaCaT cells were transfected with the ARE-Luc reporter and siRNA for Nrf2 together with a Renilla-luciferase vector using the DharmaFECT® Duo transfection reagent (Thermo Fischer Scientific, Waltham, MA, USA). After incubation for 24 h, the cells were treated with resorcinol (3 mM) under serum-free conditions for 14 h. The cells were then subjected to luciferase reporter assay. *p<0.05 vs. resorcinol-treated control. Three biological experimental replicates were performed. Data are expressed as mean ± SD. (B-D) HaCaT cells were transfected with siRNA for Nrf2 using the DharmaFECT® Duo transfection reagent (Thermo Fischer Scientific). After incubation for 24 h, the cells were incubated with resorcinol (3 mM) under serum-free conditions for 14 h. These cells were then subjected to western blot (B, D) and real-time PCR (C) analyses for Nrf2, NQO1, and HO-1. *p<0.05 vs. resorcinol-treated control. Three biological experimental replicates were performed. Data are expressed as mean ± SD. RES: resorcinol.
Biomolecules & Therapeutics 2021;29:227~233 https://doi.org/10.4062/biomolther.2020.083
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