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Fig. 3. Resorcinol activates Nrf2-mediated signaling. (A) HaCaT cells were transfected with the ARE-Luc reporter together with a Renilla-luciferase vector using the DharmaFECT® Duo transfection reagent (Thermo Fischer Scientific, Waltham, MA, USA). After incubation for 24 h, the cells were treated with resorcinol under serum-free conditions for 14 h followed by luciferase reporter assay. *p<0.05 vs. untreated control. Three biological experimental replicates were performed. Data are expressed as mean ± SD. (B-D) HaCaT cells were incubated with resorcinol for 24 h and subjected to western blot analysis (B) and real-time PCR analysis (C) for Nrf2, NQO1, and HO-1, and nuclear fractionation to assess Nrf2 translocation (D). Three biological experimental replicates were performed. *p<0.05 vs. untreated control. RES: resorcinol.
Biomolecules & Therapeutics 2021;29:227~233 https://doi.org/10.4062/biomolther.2020.083
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