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Fig. 2. Resorcinol reduces B[a]P-induced production of ROS and IL-8 in HaCaT cells. HaCaT cells were incubated with resorcinol in the presence of B[a]P (15 μM) for 24 h, then subjected to fluorescence image analysis (A) and fluorescence intensity analysis (B). Three biological experimental replicates were performed. Data are expressed as mean ± SD. *p<0.05 vs. B[a]P-treated control. (C) HaCaT cells were transfected with siRNA for the AhR gene using the DharmaFECT® Duo transfection reagent (Thermo Fischer Scientific, Waltham, MA, USA). After incubation for 24 h, the cells were incubated with resorcinol (3 mM) in the presence of B[a]P (15 μM) for 24 h and then subjected to ELISA for IL-8 (C) and Western blot analysis for AhR (D). Three biological experimental replicates were performed. Data are expressed as mean ± SD. *p<0.05 vs. B[a]P-treated control. RES: resorcinol, NAC: N-acetyl cysteine.
Biomolecules & Therapeutics 2021;29:227~233 https://doi.org/10.4062/biomolther.2020.083
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