Fig. 6. Aphidicolin also increases NO production and eNOS expression through activation of ATM/Akt/CREB/eNOS signaling pathway in HUVECs. (A) HUVECs were treated with 20 µM aphidicolin or vehicle (DMSO) for 24 h and level of NO production was measured as described in
Fig. 1. (B) HUVECs were treated with 20 µM aphidicolin or vehicle (DMSO) for 24 h, and then levels of eNOS, p-ATM-Ser
1981, p-Akt-Ser
473, or p-CREB-Ser
133 were measured using western blot analysis as described in
Fig. 1. (B-C4) Reprobing and quantitation of eNOS, p-ATM-Ser
1981, p-Akt-Ser
473, and p-CREB-Ser
133 was done as described in
Fig. 1. All experiments were performed at least four times independently (n=4). Bar graphs depict mean fold alterations above the controls (± SD). Statistical significance was evaluated using Student’s
t test. All differences were considered to be statistically significant at a
p value of <0.05. *
p<0.05 and **
p<0.01.
