Table 2 Primers used for detecting specific botulinum toxin genes
| Types | Genes | Primer sequences (5’-3’) |
|---|---|---|
| Type A | BT(A) | |
| Forward | AGCTACGGAGGCAGCTATGTT | |
| Reverse | CGTATTTCCAAAGCTGAAAAGG | |
| Type B | BT(B) | |
| Forward | CAGGAGAAGTGGAGCGAAAA | |
| Reverse | CTTGCGCCTTTGTTTTCTTG | |
| Type C | BT(C) | |
| Forward | CCAAGATTTTCATCCGCCTA | |
| Reverse | GCTATTGATCCAAAACGGTGA | |
| Type D | BT(D) | |
| Forward | CGGCTTCATTAGAGAACGGA | |
| Reverse | TAACTCCCCTAGCCCCGTAT | |
| Type E | BT(E) | |
| Forward | CCAAGATTTTCATCCGCCTA | |
| Reverse | GCTATTGATCCAAAACGGTGA | |
| Type F | BT(F) | |
| Forward | CGGCTTCATTAGAGAACGGA | |
| Reverse | TAACTCCCCTAGCCCCGTAT | |
BT, Botulinum toxin.