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Fig. 2. Schematic overview of immune-detection of toxin-cleaved SNAP-25. Toxin A cleaves SNAP-25 in neurons between the 197th and 198th amino acids, and toxin E cleaves between the 180th and 181st amino acids. The cleaved fragments bind to their specific antibodies and are caught by neo-epitope antibodies produced against the peptides corresponding to SNAP-25190-197 and SNAP-25173-180. The antibodies only detect the toxin-cleaved SNAP-25 fragment and would not bind to intact SNAP-25. The captured cleavage product is then detected using two polyclonal detection antibodies that bind to two distinct sites, SNAP-251-57 and SNAP-25111-157.
Biomolecules & Therapeutics 2020;28:302~310 https://doi.org/10.4062/biomolther.2019.200
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