Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 3. Effect of MHY4381 or SAHA on cell cycle distribution in prostate cancer cells (DU145, LNCaP, and PC-3). (A) Cells were treated with MHY4381 or SAHA at the indicated concentrations for 48 h, stained with propidium iodide (PI) and analyzed by flow cytometry to determine the cell cycle distribution. (B) Bar diagram showing the G1, S, and G2/M phase distributions of prostate cancer cells treated with vehicle control, MHY4381, or SAHA. Data are expressed as mean (n=3). (C) Effect of MHY4381 or SAHA on the protein expression levels of cell cycle regulatory proteins. DU145, LNCaP, and PC-3 cells were treated with MHY4381 (0.1, 0.5, or 1.0 μM) or SAHA (1 μM) for 48 h. The expression levels of cyclin B, cyclin A, cyclin D, p21, p27, and p53 were detected by western blot analysis. Data are representative of three independent experiments. β-Actin was used as a loading control.
Biomolecules & Therapeutics 2020;28:184~194
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