Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 3. Knockdown of G0S2 decreases cell proliferation and promotes apoptosis in MDA-MB-231 cells. MDA-MB-231 cells were treated with G0S2 siRNA for 48 h. (A) Total RNA was isolated, and qPCR was performed to confirm knockdown of G0S2 by G0S2 siRNA. All results were normalized to 18S rRNA. The data is shown as mean ± SEM (n=3). (B) Cell viability assay. The percentage of cells surviving after G0S2 siRNA treatment was calculated. (C) Colony formation assay. Cells treated with G0S2 siRNA were seeded in 6-well plates at 200 cells/well and incubated. After 48 h, the medium was replaced with fresh growth medium every 2 days. After 10 days, the cells were fixed, stained, and photographed. (D) Cell cycle analysis. Cells were treated with G0S2 siRNA for 48 h, and then, cell cycle was examined using flow cytometry. (E) Apoptosis analysis. After G0S2 siRNA treatment for 48 h, apoptosis was evaluated using flow cytometry. The plots from FACS show percentage of live, early and late apoptotic, and dead cells. The data shows percentage of total apoptotic cells which means sum of percentage of early and late apoptotic cells. All data are shown as mean ± SEM (n=3). Statistically significant differences from control when *p<0.05.
Biomolecules & Therapeutics 2019;27:591~602
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