Fig. 6. NIA protected cells from PM2.5-induced apoptosis, by blocking calcium channel and balancing mitochondrial membrane potential. (A) Fluo-4-AM staining was used to determine the intracellular Ca2+ levels. (B) Δψm analysis was performed using JC-1 dye and confocal microscopy. (C) Apoptotic bodies (indicated with arrows) were visualized using Hoechst 33342 dye. *p<0.05 or #p<0.05 compared with control cells or PM2.5-exposed cells, respectively.
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