Fig. 2. NIA cleared ROS by inhibiting NOX activity induced by PM2.5. (A) The ratio of intracellular NADP and NADPH was assessed using NADP/NADPH assay kit. (B) Intracellular ROS was detected after staining of cells with DCF-DA dye. (C) Superoxide generation was detected after dying cells with DHE. NIA diminished superoxide levels induced by PM2.5. *p<0.05 and #p<0.05 compared with control cells and PM2.5-exposed cells, respectively.
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