Fig. 5. Effects on neuronal mitochondrial respiration and cell viability by culture-conditioned medium from senescent astrocytes. (A) The cell viability of ACM-treated neurons after H2O2 stimulation was determined by MTT assay. Values are expressed as the mean ± SEM. * indicates p<0.05, and *** indicates p<0.001 vs. no ACM group. (B) Mitochondrial oxygen consumption rate (OCR) was measured in astrocytes by Cell Mito Test Kit (Agilent Technology) and Agilent Seahorse XFe96 Analyzer (Agilent Technology). The representative graph was obtained using Wave Desktop 2.6 software (Agilent Technology). (C, D) The basal level, spare respiratory capacity, proton leak, and ATP production level in neurons treated with ACM from senescent astrocytes.
© Biomolecules & Therapeutics
