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Fig. 3. Effect of poncirin on osteoclast-specific gene expression in sRANKL-stimulated RAW264.7 cells. (A) Cells were pretreated with the indicated concentration of poncirin, followed by culturing with sRANKL (100 ng/mL) for 4 days. The mRNA levels of TRAP, MMP9, and cathepsin K were evaluated by quantitative real-time PCR. (B) Cells were cultured with sRANKL (100 ng/mL) for 1 day, and then further incubated in the presence of the indicated concentration of poncirin for additional 3 days; the mRNA level of TRAP was determined by quantitative real-time PCR. Fold changes relative to each gene level in the control are presented as mean ± SD of independent experiments (n=3). ###p<0.001 compared with the control. **p<0.01, ***p<0.001 compared with sRANKL.
Biomol Ther 2020;28:337~343
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