Biomol Ther  
Gomisin G Suppresses the Growth of Colon Cancer Cells by Attenuation of AKT Phosphorylation and Arrest of Cell Cycle Progression
Sony Maharjan1,†, Byoung Kwon Park1,†, Su In Lee1, Yoongho Lim2, Keunwook Lee3, Younghee Lee4 and Hyung-Joo Kwon1,5,*
1Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252,
2Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul 05029,
3Department of Biomedical Science, College of Natural Science, Hallym University, Chuncheon 24252,
4Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju 28644,
5Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea
E-mail: hjookwon@hallym.ac.kr
Tel: +82-33-248-2635, Fax: +82-33-241-3640
The first two authors contributed equally to this work.
Received: March 26, 2018; Revised: May 1, 2018; Accepted: May 17, 2018; Published online: June 14, 2018.
© The Korean Society of Applied Pharmacology. All rights reserved.

Abstract
Colorectal cancer is one of the leading causes of cancer related death due to a poor prognosis. In this study, we investigated the effect of Gomisin G on colon cancer growth and examined the underlying mechanism of action. We found that Gomisin G significantly suppressed the viability and colony formation of LoVo cells. Gomisin G reduced the phosphorylation level of AKT implying that Gomisin G suppressed the PI3K-AKT signaling pathway. Gomisin G also induced apoptosis shown by Annexin V staining and an increased level of cleaved poly-ADP ribose polymerase (PARP) and Caspase-3 proteins. Furthermore, Gomisin G remarkably triggered the accumulation of cells at the sub-G1 phase which represents apoptotic cells. In addition, the level of cyclin D1 and phosphorylated retinoblastoma tumor suppressor protein (Rb) was also reduced by the treatment with Gomisin G thus curtailing cell cycle progression. These findings show the suppressive effect of Gomisin G by inhibiting proliferation and inducing apoptosis in LoVo cells. Taken together, these results suggest Gomisin G could be developed as a potential therapeutic compound against colon cancer.
Keywords: Gomisin G, Colon cancer, AKT, Apoptosis, PARP, Cell cycle


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