Biomol Ther  
Construction of a Transcriptome-Driven Network at the Early Stage of Infection with Influenza A H1N1 in Human Lung Alveolar Epithelial Cells
Myungguen Chung1,†, Soo Young Cho2,† and Young Seek Lee1,*
1Division of Molecular and Life Sciences, Hanyang University, Ansan 15588,
2National Cancer Center, Goyang 10408, Republic of Korea
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The first two authors contributed equally to this work.
Received: December 13, 2017; Revised: December 29, 2017; Accepted: January 2, 2018; Published online: February 5, 2018.
© The Korean Society of Applied Pharmacology. All rights reserved.

We aimed to understand the molecular changes in host cells that accompany infection by the seasonal influenza A H1N1 virus because the initial response rapidly changes owing to the fact that the virus has a robust initial propagation phase. Human epithelial alveolar A549 cells were infected and total RNA was extracted at 30 min, 1 h, 2 h, 4 h, 8 h, 24 h, and 48 h post infection (h.p.i.). The differentially expressed host genes were clustered into two distinct sets of genes as the infection progressed over time. The patterns of expression were significantly different at the early stages of infection. One of the responses showed roles similar to those associated with the enrichment gene sets to known ‘gp120 pathway in HIV.’ This gene set contains genes known to play roles in preventing the progress of apoptosis, which infected cells undergo as a response to viral infection. The other gene set showed enrichment of ‘Drug Metabolism Enzymes (DMEs).’ The identification of two distinct gene sets indicates that the virus regulates the cell’s mechanisms to create a favorable environment for its stable replication and protection of gene metabolites within 8 h.
Keywords: A549 Cells, Influenza A virus H1N1 subtype, High-throughput nucleotide sequencing, Sequence analysis RNA, Apoptosis, Gene expression regulation

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