Biomol Ther (Seoul)  
Chemical Constituents Identified from Fruit Body of Cordyceps bassiana and Their Anti-Inflammatory Activity
Wonse Suh1,†, Gyeongsug Nam2,†, Woo Seok Yang2,†, Gi-Ho Sung3, Sang Hee Shim4,* and Jae Youl Cho2,*
1School of Biotechnology, Yeungnam University, Gyeongsan 38541,
2Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419,
3Institute for Bio-Medical Convergence, International St. Mary’s Hospital and College of Medicine, Catholic Kwandong University, Incheon 22711,
4College of Pharmacy, Duksung Women’s University, Seoul 01369, Republic of Korea
E-mail: sangheeshim@duksung.ac.kr (Shim SH), jaecho@skku.edu (Cho JY)
Tel: +82-2-901-8774 (Shim SH), +82-31-290-7868 (Cho JY)
Fax: +82-2-901-8386 (Shim SH), +82-31-290-7870 (Cho JY)
The first three authors contributed equally to this work.
Received: March 21, 2016; Revised: May 5, 2016; Accepted: June 20, 2016; Published online: August 19, 2016.
© The Korean Society of Applied Pharmacology. All rights reserved.

Abstract
Cordyceps bassiana is one of Cordyceps species with anti-oxidative, anti-cancer, anti-inflammatory, anti-diabetic, anti-obesity, anti-angiogenic, and anti-nociceptive activities. This mushroom has recently demonstrated to have an ability to reduce 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in NC/Nga mice. In this study, we further examined phytochemical properties of this mushroom by column chromatography and HPLC analysis. By chromatographic separation and spectroscopic analysis, 8 compounds, such as 1,9-dimethylguanine (1), adenosine (2), uridine (3), nicotinamide (4), 3-methyluracil (5), 1,7-dimethylxanthine (6), nudifloric acid (7), and mannitol (8) were identified from 6 different fractions and 4 more subfractions. Through evaluation of their anti-inflammatory activities using reporter gene assay and mRNA analysis, compound 1 was found to block luciferase activity induced by NF-κB and AP-1, suppress the mRNA levels of cyclooxygenase (COX)-2 and tumor necrosis factor (TNF)-a. Therefore, our data strongly suggests that compound 1 acts as one of major principles in Cordyceps bassiana with anti-inflammatory and anti-atopic dermatitis activities.
Keywords: Cordyceps bassiana, Phytochemical study, 1,9-dimethylguanine, NF-κB, AP-1, Anti-inflammatory activity


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