Biomolecules & Therapeutics 2020; 28(1): 104-109  https://doi.org/10.4062/biomolther.2019.047
Inhibitory Effect of Probenecid on Osteoclast Formation via JNK, ROS and COX-2
Mi Hyun Cheng and Sung-Jin Kim*
Department of Pharmacology and Toxicology, School of Dentistry, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea
*E-mail: kimsj@khu.ac.kr
Tel: +82-2-961-0868
Received: March 13, 2019; Revised: July 9, 2019; Accepted: August 9, 2019; Published online: September 2, 2019.
© The Korean Society of Applied Pharmacology. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

Probenecid is a representative drug used in the treatment of gout. A recent study showed that probenecid effectively inhibits oxidative stress in neural cells. In the present study, we investigated whether probenecid can affect osteoclast formation through the inhibition of reactive oxygen species (ROS) formation in RAW264.7 cells. Lipopolysaccharide (LPS)-induced ROS levels were dose-dependently reduced by probenecid. Fluorescence microscopy analysis clearly showed that probenecid inhibits the generation of ROS. Western blot analysis indicated that probenecid affects two downstream signaling molecules of ROS, cyclooxygenase 2 (COX-2) and c-Jun N-terminal kinase (JNK). These results indicate that probenecid inhibits ROS generation and exerts antiosteoclastogenic activity by inhibiting the COX-2 and JNK pathways. These results suggest that probenecid could potentially be used as a therapeutic agent to prevent bone resorption.

Keywords: Probenecid, Antiosteoclastogenesis, Oxidative stress, ROS, COX-2, JNK
INTRODUCTION

Osteoblasts and osteoclasts work together to control the amount of total bone mass by regulating bone remodeling. It has been suggested that pathological bone diseases such as rheumatoid arthritis and osteoporosis are caused by excessive osteoclast differentiation (Boyle et al., 2003). Receptor activator of nuclear factor kappa-B ligand (RANKL) plays an important role in osteoclast activation and differentiation (Yasuda et al., 1998; Suda et al., 1999; Teitelbaum and Ross, 2003). Various signaling molecules including nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), extracellular signal-regulated kinase (ERK), PI3K/Akt, c-Jun N-terminal kinase (JNK) and cyclooxygenase 2 (COX-2) are involved in osteoclastogenesis (Darnay et al., 1999; Matsumoto et al., 2000; Zhang et al., 2001; Hou et al., 2013; Mizutani et al., 2013; Chen et al., 2019).

Cell damage due to oxidative stress is associated with a diverse range of metabolic diseases including osteoporosis, diabetes and neurodegenerative disorders like Alzheimer’s disease. Reactive oxygen species (ROS) are the most important mediator of cell damage caused by oxidative stress. The ROS include superoxide radical (O2), hydroxyl radical (OH•), hydrogen peroxide (H2O2) and peroxynitrate (ONOO), which occur when DNA damage, oxidative burst, cell lysis, protein oxidation, lipid peroxidation, excitatory amino acids and cell death including apoptosis (Chandra et al., 2000; Ruffels et al., 2004; Zhang et al., 2007; Zhuang et al., 2007; Chen et al., 2009; Lin et al., 2009). Various cytokines and growth factors, such as TNF-α, are known to bind to receptors to produce ROS, which triggers an increase in ROS levels in a variety of cells (Thannickal and Fanburg, 2000). High levels of ROS in cells can lead to inflammatory reactions, aging, apoptosis and cancer. In contrast, low levels of ROS can serve as secondary messengers in various signaling pathways (Sundaresan et al., 1995; Bae et al., 1997; Forman et al., 2004). Studies have been conducted on the role of ROS in osteoclast differentiation (Lee et al., 2005). Osteoclasts have been found to be highly susceptible to oxidative stress, and they are activated and differentiated by ROS (Garrett et al., 1990; Steinbeck et al., 1994; Fraser et al., 1996).

Probenecid has long been used as a treatment for gout. It inhibits the renal tubular transporter to prevent the reuptake of uric acid, thereby promoting the excretion of uric acid (Gutman, 1951; Beachwood et al., 1964; Moller, 1965). In a recent study, probenecid was shown to exhibit antihypertensive action by inhibiting the α-adrenergic receptor (Park and Kim, 2011). Furthermore, it was found that probenecid blocks the efflux of antioxidant glutathione (GSH), as well as GSH conjugates in neurons (Du et al., 2016). Although the molecular mechanisms involved in oxidative stress-induced osteoclast differentiation are complex and not well characterized (Lee et al., 2005; Oka et al., 2012), therapeutic strategies to prevent ROS generation may be useful in overcoming many diseases, including osteoporosis and diabetes. Here, we investigate whether probenecid has the potential to regulate osteoclastogenesis by inhibiting oxidative stress. The objective of this study was to evaluate whether probenecid inhibits lipopolysaccharide (LPS)-induced osteoclast formation by inhibiting ROS production using RAW264.7 cells.

MATERIALS AND METHODS

Reagents and materials

Ethylenediaminetetraacetic acid disodium salt (Na2-EDTA), sodium azide, LPS and 2,7-dichlorofluorescin diacetate (DCF-DA) were purchased from Sigma Chemical Co (St. Louis, MO, USA). The materials needed for cell culture were obtained from Gibco BRL (Gaithersburg, MD, USA), and VECTASHIELD mounting medium for fluorescence staining with DAPI was purchased from Vector Laboratories Inc (Burlingame, CA, USA). JNK, COX-2 and GAPDH antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The ECL kit was purchased from Amersham BioScience (Amersham, UK) and probenecid was obtained from Sigma Chemical Co.

Cell culture

The RAW264.7 cell line was purchased from American Type Culture Collection (ATCC; ATCC®TIB-71, Manassas, VA, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, NY, USA) containing 10% fetal bovine serum (FBS; Gibco), penicillin (100 U/mL; Gibco) and streptomycin (100 μg/mL; Gibco) in a humidified incubator with 5% CO2 at 37°C. Cells were cultured in a six-well plate at a density of 106 for 24 h prior to probenecid treatment. After cells had grown to confluence, they were incubated in a serum-free starvation state. Serum-free starvation cells were incubated with probenecid at four different concentrations (0.1, 1, 10 and 100 μM) for 4 h, then treated with LPS (10 μg/mL) for 24 h. The cells were subjected to western blot analysis.

Detection of intracellular reactive oxygen species

Intracellular ROS were detected using 2,7-dichlorofluorescin diacetate (DCF-dAc), a membrane-permeable tracer that is oxidized by various types of ROS. For this, 100 μM of DCF-dAc was added to cells that had been cultured as described above, and incubated in the dark for 30 min at 37°C. The cells were washed twice with phosphate-buffered saline (PBS). Fluorescence was measured using a fluorescent microplate reader (TRIAD Series Multimode Detector; Dynex Technologies, Inc., VA, USA) with an excitation wavelength of 485 nm and an emission wavelength of 530 nm.

Intracellular reactive oxygen species staining

To assess intracellular ROS staining, 100 μM of DCFH-DA was added to cells cultured as described above, and incubated in the dark for 30 min at 37°C. The cells were washed three times with PBS then mounted on glass slides using aqueous mounting medium containing DAPI. DCF and DAPI fluorescence were detected using an Olympus BX50 microscope (Olympus, Tokyo, Japan) and images were acquired using an Olympus DP71 camera (Olympus).

Western blot analysis

The cells were washed three times with PBS, scraped off, then centrifuged at 12,000 rpm for 5 min. Cells were collected and lysed as previously described (Jang and Kim, 2013). The concentration of total protein was quantified using a Bradford assay (Bio-Rad, Hercules, CA, USA). Samples with equal amounts of protein (50 μg) were subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) then transferred to a nitrocellulose membrane. To prevent non-specific binding, membranes were incubated in 5% skim milk for 1 h. After blocking, each membrane was incubated with antibodies against pJNK (1:1,000; Santa Cruz Biotechnology), COX-2 (1:2,000; Santa Cruz Biotechnology) and GAPDH (1:1,000; Santa Cruz Biotechnology). The blots were detected using a chemiluminescence detection system (ECL, Amersham BioScience).

Ileum contractile analysis

The contraction of rat ileum was measured as previously described (Schapoval et al., 1998) but with slight modifications. The contraction response was constantly recorded in an isotonic solution using Grass Low Level DC Amplifier (model 7P122P). After 30 min of equilibration, concentration-response curves were generated by placing the contractile agonist (bradykinin, 10 μM) in the organ bath. After washing the ileum and restoring baseline tension, probenecid (1 μM) was added to the organ bath and incubated for 15 min. Bradykinin was added and the contraction reaction was recorded using MacLab 8E data acquisition system (AD Instruments, Vella Vista, New South Wales, Australia).

Tartrate-resistant acid phosphatase assay

After culturing cells according to the above method and washing with PBS, a tartrate-resistant acid phosphatase (TRAP) assay was performed according to the manufacturer’s instructions (Sigma Chemical Co.). AP assay buffer was added to the cells and incubated at 37°C for 2 h. The AP stop buffer was added to stop the reaction, and the absorbance was read at 410 nm with a TRIAD Series Multimode Detector (Dynex Technologies Inc.).

Statistical analysis

All experiments were performed in triplicate, and the results are expressed as the mean ± standard deviation. Data were analyzed using GraphPad Prism 5 software (GraphPad software, La Jolla, CA, USA). One-way ANOVA and Tukey’s HSD post hoc test were used. Differences were considered statistically significant at p<0.05.

RESULTS

Probenecid inhibits lipopolysaccharide-induced production of reactive oxygen species in RWA264.7 cells

Untreated RAW264.7 cells showed increased LPS-induced ROS production, while probenecid treatment suppressed ROS production in a dose-dependent manner (Fig. 1). The fluorescence analysis confirmed that LPS-induced ROS production was reversed by probenecid (Fig. 2). The concentration of probenecid with the greatest ROS inhibition was 100 μM.

Effect of probenecid on signaling molecules downstream of reactive oxygen species

To investigate the mechanism by which probenecid inhibits the ROS induced by LPS, western blot analysis was performed using antibodies specific to JNK and COX-2. While the phosphorylation of JNK (pJNK) was dramatically induced by LPS, it was markedly decreased when pretreated with probenecid, and the maximum inhibitory concentration of probenecid was 100 μM (Fig. 3).

Effect of probenecid on bradykinin-mediated rat ileum contraction

Isolated rat ileum is a sensitive bioassay tissue for testing biomolecules with antagonistic effects on the bradykinin B1 receptor (Vietinghoff et al., 2003), therefore we assessed whether probenecid could affect the binding of bradykinin to its B1 receptor by employing rat ileum contraction experiments. Probenecid did not show any effect on bradykinin binding (Fig. 4).

Probenecid inhibits osteoclast formation in RAW264.7 cells

To confirm whether probenecid affects LPS-induced osteoclastogenesis, a TRAP assay was performed. Probenecid significantly inhibited LPS-induced osteoclast differentiation, and the highest inhibitory concentration was 100 μM (Fig. 5).

DISCUSSION

Probenecid has been studied for many decades and is known to exhibit various pharmacological actions. In this study, we examined whether probenecid could affect osteoclastogenesis and whether ROS, JNK and COX-2 are involved in the action of probenecid. Clinical studies have shown that probenecid is effective in reducing serum uric acid levels. It has been used as a treatment for gout for decades, and has been shown to improve the symptoms of gout patients (Talbott, 1951; Sirota et al., 1952; Bishop and Pfaff, 1955; Boger and Strickland, 1955).

In addition to increasing uric acid excretion, probenecid inhibits the α-adrenergic receptor, exhibiting an antihypertensive effect. Interestingly, probenecid also interferes with the efflux of GSH and GSH conjugates in neurons, suggesting the possibility of an antioxidative effect on osteoclast differentiation. Therefore, it is reasonable to assume that the ability of probenecid to interfere in oxidative stress could mean that it also affects osteoclast differentiation. We present evidence that probenecid inhibits osteoclastogenesis by inhibiting JNK phosphorylation, ROS production and COX-2 expression. It is well known that intracellular ROS are generated during the process of osteoclastogenesis. The increased level of ROS also promotes osteoclastogenesis and plays an important role in the signaling process involved in osteoclast activation, resulting in bone resorption (Hall et al., 1995). In the present study, we provide evidence that probenecid inhibits LPS-induced ROS production in RAW264.7 cells, suggesting that ROS could be a target of probenecid action. However, the reduction in ROS production by probenecid was not dramatic, indicating that it might play a minor role in the inhibition of osteoclastogenesis.

Considering that ROS requires an activated JNK, we attempted to determine whether JNK activity could be influenced by probenecid. As shown in Fig. 3, LPS-induced phosphorylation of JNK was significantly reduced by probenecid, indicating that it is an important molecular target in the antiosteoclastogenic activity of probenecid. LPS has been reported to increase osteoclastogenesis through the activation of JNK and increased expression of COX-2 (Hou et al., 2013), suggesting that JNK phosphorylation and COX-2 expression could be essential regulators of osteoclast differentiation.

In this study, we examined the effect of probenecid on the COX-2 signaling pathway. As shown in Fig. 3, the LPS-induced expression of COX-2 was inhibited by probenecid at concentrations ranging from 0.1 to 100 μM. However, there was a small increase in the expression of COX-2 in cells treated with 1 to 10 μM of probenecid when compared to those treated with 0.1 μM. COX-2 is a rate-limiting enzyme for prostaglandin synthesis, and also undergoes feedback control depending on changes in its expression (Tang et al., 2017). It is possible that probenecid at concentrations of 0.1 to 10 μM may have some effect on the signaling mediators involved in the feedback loop of COX-2 expression, thereby inducing slight increases in COX-2 expression under LPS treatment as the concentration increases from 0.1 to 10 μM. Nevertheless, probenecid inhibits LPS-induced COX-2 expression, supporting its antiosteoclastogenic action.

Bradykinin has been found to increase mitochondrial ROS production in cell culture (Greene et al., 2000; Oldenburg et al., 2004), inducing inflammation. Bradykinin receptors (B1 and B2) are expressed in smooth muscle cells, and bradykinin has a higher affinity for the B1 receptor than the B2 receptor. B2 receptors are constitutively expressed in many tissues whereas B1 receptors are induced under conditions of tissue injury, such as inflammation and oxidative stress (Vietinghoff et al., 2003). Isolated rat ileum is widely used to investigate potential antagonistic effects on the bradykinin B1 receptor. In the present study, probenecid had no effect on bradykinin-induced rat ileum contraction, suggesting that probenecid may not directly interfere in the binding of bradykinin to B1 receptors. A TRAP assay was performed to confirm that RAW264.7 cells were differentiated into osteoclasts by LPS. Treatment with probenecid blocked osteoclast differentiation, confirming that probenecid inhibited osteoclast differentiation (Fig. 5). A recent study showed that the COX-2 inhibitor, celecoxib, inhibits RANK expression, and thus inhibits osteoclast differentiation (Geng et al., 2011). Therefore, it is reasonable to assume that probenecid inhibits osteoclast formation by inhibiting COX-2 and RANK expression. We took all measurents 24 h after stimuli, since we and other investigatiors have previously found that ROS production and JNK activation by LPS could last for 24 h (Jang and Kim, 2013; Hwang et al., 2016). Considering the early activation of JNK, we depicted a signaling cascade of JNK - ROS -NFκB - COX2 in Fig. 6. Our proposed mechanism of action is shown in Fig. 6. It will be necessary to explore whether other signaling proteins that are important for osteoclastogenesis, such as cathepsin K, calcitonin receptor, TNF receptor-associated factor 6 and matrix metalloproteinase 9, are involved in the action of probenecid.

In conclusion, we found an effect of probenecid on osteoclast formation, and the mechanism of action of probenecid may involve the inhibition of JNK phosphorylation, ROS formation, and the subsequent blocking of COX-2 expression. Currently available drugs for the treatment of metabolic bone diseases include bisphosphonates, denosumab and selective estrogen receptor modulators. However, these drugs have serious side effects such as osteonecrosis of the jaws and increased endometrial cancer risk (Wu et al., 2018; Zhang et al., 2018), highlighting the necessity for novel drug development. Taken together, our results suggest that probenecid could potentially be used as a new therapeutic agent for the treatment of metabolic bone diseases such as osteoarthritis, osteoporosis and rheumatic arthritis.

Figures
Fig. 1. Probenecid inhibits lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) production in RAW264.7 cells. RAW264.7 cells grown in serum-free medium were incubated with probenecid (0.1, 1, 10 and 100 μM) for 4 h, then cultured for 24 h after LPS (10 μg/mL) treatment. Measurement of ROS in cells was performed using a microplate reader, as described in the “Materials and Methods” section. Data are presented as the mean ± standard deviation of four experiments performed in triplicate. *p<0.05 compared to the LPS-treated group.
Fig. 2. Detection of intracellular reactive oxygen species (ROS) induced by lipopolysaccharide (LPS) in the presence of probenecid in LPS-treated RAW264.7 cells. RAW264.7 cells grown in serum-free medium were incubated with probenecid (0.1, 1, 10 and 100 μM) for 4 h, then cultured for 24 h after LPS (10 μg/mL) treatment. Assessment of ROS by microscopy was performed as described in the “Materials and Methods” section.
Fig. 3. Western blot analysis of pJNK and COX-2 in response to probenecid in lipopolysaccharide (LPS)-treated RAW264.7 cells. RAW264.7 cells grown in serum-free medium were incubated with probenecid (0.1, 1, 10 and 100 μM) for 4 h, then cultured for 24 h after LPS (10 μg/mL) treatment. The western blot procedure is described in the “Materials and Methods” section.
Fig. 4. Effect of probenecid on bradykinin-induced rat ileum contraction. Segmented rat ileum was placed in an organ bath with a load of 1 g at 37°C. The ileum was equilibrated for 30 minutes before the experiment began. Organ processing for the ileum contraction assay is described in the “Materials and Methods” section.
Fig. 5. Probenecid blocks osteoclastogenesis in RAW264.7 cells. RAW264.7 cells grown in serum-free medium were incubated with probenecid (0.1, 1, 10 and 100 μM) for 4 h, then cultured for 24 h after LPS (10 μg/mL) treatment. The subsequent TRAP assay procedure is described in the “Materials and Methods” section. Data are presented as the mean ± standard deviation of four experiments performed in triplicate. ***p<0.001 compared to the LPS-treated group.
Fig. 6. Schematic illustration of the proposed mechanism of probenecid in lipopolysaccharide (LPS)-induced RAW264.7 cells. Probenecid exhibits antiosteoclastogenic activity by inhibiting reactive oxygen species (ROS) formation, JNK phosphorylation and COX-2 expression.
References
  1. Bae, YS, Kang, SW, Seo, MS, Baines, IC, Tekle, E, Chock, PB, and Rhee, SG (1997). Epidermal growth factor (EGF)-induced generation of hydrogen peroxide. Role in EGF receptor-mediated tyrosine phosphorylation.. J. Biol. Chem.. 272, 217-221.
    Pubmed CrossRef
  2. Beachwood, EC, Berndt, WO, and Mudge, GH (1964). Stop-flow analysis of tubular transport of uric acid in rabbits. Am. J. Physiol. 207, 1265-1272.
    Pubmed CrossRef
  3. Bishop, C, and Pfaff, W (1955). Immediate uricosuric effect of probenecid in normal humans. Proc. Soc. Exp. Biol. Med. 88, 346-348.
    Pubmed CrossRef
  4. Boger, WP, and Strickland, SC (1955). Probenecid (benemid); Its uses and side-effects in 2,502 patients. AMA Arch. Intern. Med. 95, 83-92.
    Pubmed CrossRef
  5. Boyle, WJ, Simonet, WS, and Lacey, DL (2003). Osteoclast differentiation and activation. Nature. 423, 337-342.
    Pubmed CrossRef
  6. Chandra, J, Samali, A, and Orrenius, S (2000). Triggering and modulation of apoptosis by oxidative stress. Free Radic. Biol. Med. 29, 323-333.
    Pubmed CrossRef
  7. Chen, L, Liu, L, Yin, J, Luo, Y, and Huang, S (2009). Hydrogen peroxide-induced neuronal apoptosis is associated with inhibition of protein phosphatase 2A and 5, leading to activation of MAPK pathway. Int. J. Biochem. Cell Biol. 41, 1284-1295.
    Pubmed CrossRef
  8. Chen, G, Xu, Q, Dai, M, and Liu, X (2019). Bergapten suppresses RANKL-induced osteoclastogenesis and ovariectomy-induced osteoporosis via suppression of NF-kB and JNK signaling pathways. Biochem. Biophys. Res. Commun. 509, 329-334.
    Pubmed CrossRef
  9. Darnay, BG, Ni, J, Moore, PA, and Aggarwal, BB (1999). Activation of NK-kappaB by RANK requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase. Identification of a novel TRAF6 interaction motif.. J. Biol. Chem.. 274, 7724-7777.
    Pubmed CrossRef
  10. Du, L, Empey, PE, Ji, J, Chao, H, Kochanek, PM, Bayır, H, and Clark, RS (2016). Probenecid and N-Acetylcysteine prevent loss of intracellular glutathione and inhibit neuronal death after mechanical stretch injury in vitro. J. Neurotrauma. 33, 1913-1917.
    Pubmed KoreaMed CrossRef
  11. Forman, HJ, Fukuto, JM, and Torres, M (2004). Redox signaling: Thiol chemistry defines which reactive oxygen and nitrogen species can act as second messengers. Am. J. Physiol. Cell Physiol. 287, 246-256.
    Pubmed CrossRef
  12. Fraser, JH, Helfrich, MH, Wallace, HM, and Ralston, SH (1996). Hydrogen peroxide, but not superoxide, stimulates bone resorption in mouse calvariae. Bone. 19, 223-226.
    Pubmed CrossRef
  13. Garrett, IR, Boyce, BF, Oreffo, RO, Bonewald, L, Poser, J, and Mundy, GR (1990). Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro in vivo. J. Clin. Invest. 85, 632-639.
    Pubmed KoreaMed CrossRef
  14. Geng, DG, Zhu, XS, Mao, HQ, Meng, B, Chen, L, Yang, HL, and Xu, YZ (2011). Protection against titanium particle-induced osteoclastogenesis by cyclooxygenase-2 selective inhibitor. J. Biomed. Mater. Res. 99, 516-522.
    Pubmed CrossRef
  15. Greene, EL, Velarde, V, and Jaffa, AA (2000). Role of reactive oxygen species in bradykinin-induced mitogen-activated protein kinase and c-fos induction in vascular cells. Hypertension. 35, 942-947.
    Pubmed CrossRef
  16. Gutman, AB (1951). Some recent advances in the study of uric acid metabolism and gout. Bull. N. Y. Acad. Med. 27, 144-164.
    Pubmed KoreaMed
  17. Hall, TJ, Schaeublin, M, Jeker, H, Fuller, K, and Chambers, TJ (1995). The role of reactive oxygen intermediates in osteoclastic bone resorption. Biochem. Biophys. Res. Commun. 207, 280-287.
    Pubmed CrossRef
  18. Hou, GQ, Guo, C, Song, GH, Fang, N, Fan, WJ, Chen, XD, Yuan, L, and Wang, ZQ (2013). Lipopolysaccharide (LPS) promotes osteoclast differentiation and activation by enhancing the MAPK pathway and COX-2 expression in RAW264.7 cells. Int. J. Mol. Med. 32, 503-510.
    Pubmed CrossRef
  19. Hwang, JS, Ham, SA, Yoo, T, Lee, WJ, Paek, KS, Kim, JH, Lee, CH, and Seo, HG (2016). Upregulation of MKP-7 in response to rosiglitazone treatmentameliorates lipopolysaccharide-induced destabilization of SIRT1 byinactivating JNK. Pharmacol. Res. 114, 47-55.
    Pubmed CrossRef
  20. Jang, HJ, and Kim, SJ (2013). Taurine exerts anti-osteoclastogenesis activity via inhibiting ROS generation, JNK phosphorylation and COX-2 expression in RAW264.7 cells. J. Recept. Signal Transduct. Res. 33, 387-391.
    Pubmed CrossRef
  21. Lee, NK, Choi, YG, Baik, JY, Han, SY, Jeong, DW, Bae, YS, Kim, N, and Lee, SY (2005). A crucial role for reactive oxygen species in RANKL-induced osteoclast differentiation. Blood. 106, 852-859.
    Pubmed CrossRef
  22. Lin, YC, Huang, YC, Chen, SC, Liaw, CC, Kuo, SC, Huang, LJ, and Gean, PW (2009). Neuroprotective effects of Ugonin K on hydrogen peroxide-induced cell death in human neuroblastoma SH-SY5Y cells. Neurochem. Res. 34, 923-930.
    Pubmed CrossRef
  23. Matsumoto, M, Sudo, T, Saito, T, Osada, H, and Tsujimoto, M (2000). Involvement of p38 mitogenactivated protein kinase signalinf pathway in osteoclastogenesis mediated by receptor activator of NF-kappa B ligand (RANKL). J. Biol. Chem. 275, 31155-31161.
    Pubmed CrossRef
  24. Mizutani, H, Ishihara, Y, Izawa, A, Fujihara, Y, Kobayashi, S, Gotou, H, Okabe, E, Takeda, H, Ozawa, Y, Kamiya, Y, Kamei, H, Kikuchi, T, Yamamoto, G, Mitani, A, Nishihara, T, and Noguchi, T (2013). Lipopolysaccharide of Aggregatibacter actinomycetem-comitans up-regulates inflammatory cytokines, prostaglandin E2 synthesis and osteoclast formation in interleukin-1 receptor antagonist-deficient mice. J. Periodont. Res. 48, 748-756.
  25. Moller, JV (1965). The tubular site of urate transport in the rabbit kidney, and the effect of probenecid on urate secretion. Acta Pharmacol. Toxicol. (Copenh.). 23, 329-336.
    Pubmed CrossRef
  26. Oka, Y, Iwai, S, Amano, H, Irie, Y, Yatomi, K, Ryu, K, Yamada, S, Inagaki, K, and Oguchi, K (2012). Tea polyphenols inhibit rat osteoclsat formation and differentiation. J. Pharmacol. Sci. 118, 55-64.
    Pubmed CrossRef
  27. Oldenburg, I, Qin, Q, Krieg, T, Yang, XM, Philipp, S, Critz, SD, Cohen, MV, and Downey, JM (2004). Bradykinin induces mitochondrial ROS generation via NO, cGMP, PKG, and mitoKATP channel opening and leads to cardioprotection. Am. J. Physiol. Heart Circ. Physiol. 286, H468-H476.
    Pubmed CrossRef
  28. Park, JB, and Kim, SJ (2011). Anti-hypertensive effects of probenecid via inhibition of the a-adrenergic receptor. Pharmacol. Rep. 63, 1145-1150.
    Pubmed CrossRef
  29. Ruffels, J, Griffin, M, and Dickenson, JM (2004). Activation of ERK1/2, JNK and PKB by hydrogen peroxide in human SH-SY5Y neuroblastoma cells: Role of ERK1/2 in H2O2-induced cell death. Eur. J. Pharmacol. 483, 163-173.
    Pubmed CrossRef
  30. Schapoval, EE, Vargas, MR, Chaves, CG, Bridi, R, Zuanazzi, JA, and Henriques, AT (1998). Antiinflammatory and antinociceptive activities of extracts and isolated compounds from Stachytarpheta cayennensis. J. Ethnopharmacol. 60, 53-59.
    Pubmed CrossRef
  31. Sirota, JH, Yu, TF, and Gutman, AB (1952). Effect of benemid (p-[di-n-propylsulfamyl]-benzoic acid) on urate clearance and other discrete renal functions in gouty subjects. J. Clin. Invest. 31, 692-701.
    Pubmed KoreaMed CrossRef
  32. Steinbeck, MJ, Appel, WH, Verhoeven, AJ, and Karnovsky, MJ (1994). NADPH-oxidase expression and in situ production of superoxide by osteoclasts actively resorbing bone. J. Cell Biol. 126, 765-772.
    Pubmed KoreaMed CrossRef
  33. Suda, T, Takahashi, N, Udagawa, N, Jimi, E, Gillespie, MT, and Martin, TJ (1999). Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr. Rev. 20, 345-357.
    Pubmed CrossRef
  34. Sundaresan, M, Yu, ZX, Ferrans, VJ, Irani, K, and Finkel, T (1995). Requirement for generation of H2O2 for platelet-derived growth factor signal tranduction. Science. 270, 296-299.
    Pubmed CrossRef
  35. Talbott, JH (1951). Clinical and metabolic effects of benemid in gout. Bull. Rheum. Dis. 2, 1-2.
    Pubmed
  36. Tang, T, Scambler, TE, Smallie, T, Cunliffe, HE, Ross, EA, Rosner, DR, O’Neil, JD, and Clark, AR (2017). Macrophage responses to lipopolysaccharide are modulated by a feedback loop involving prostaglandin E2, dual specificity phosphatase 1 and tristetraprolin. Sci. Rep. 7, 4350.
    Pubmed KoreaMed CrossRef
  37. Teitelbaum, SL, and Ross, FP (2003). Genetic regulation of osteoclast development and function. Nat. Rev. Genet. 4, 638-649.
    Pubmed CrossRef
  38. Thannickal, VJ, and Fanburg, BL (2000). Reactive oxygen species in cell signaling. Am. J. Physiol. Lung Cell. Mol. Physiol. 279, L1005-L1028.
    Pubmed CrossRef
  39. Vietinghoff, G, Hilscher, E, Paegelow, I, and Reissmann, S (2003). Effect of bradykinin analogues on the B1 receptor of rat ileum. Peptides. 24, 931-935.
    Pubmed CrossRef
  40. Wu, H, Hu, B, Zhou, X, Zhou, C, Meng, J, Yang, Y, Zhao, X, Shi, Z, and Yan, S (2018). Artemether attenuates LPS-induced inflammatory bone loss by inhibiting osteoclastogenesis and bone resorption via suppression of MAPK signaling pathway. Cell Death Dis. 9, 498.
    Pubmed KoreaMed CrossRef
  41. Yasuda, H, Shima, N, Nakagawa, N, Yamaguchi, K, Kinosaki, M, Mochizuki, S, Tomoyasu, A, Yano, K, Goto, M, Murakami, A, Tsuda, E, Morinaga, T, Higashio, K, Udagawa, N, Takahashi, N, and Suda, T (1998). Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL. Proc. Natl. Acad. Sci. U.S.A. 95, 3597-3602.
    Pubmed KoreaMed CrossRef
  42. Zhang, L, Yu, H, Sun, Y, Lin, X, Chen, B, Tan, C, Cao, G, and Wang, Z (2007). Protective effects of salodroside on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells. Eur. J. Pharmacol. 564, 18-25.
    Pubmed CrossRef
  43. Zhang, X, Li, X, Fang, J, Hou, X, Fang, H, Guo, F, Li, F, Chen, A, and Huang, S (2018). (2R,3R)Dihydromyricetin inhibits osteoclastogenesis and bone loss through scavenging LPS-induced oxidative stress and NF-κB and MAPKs pathways activating. J. Cell. Biochem. 119, 8981-8995.
    Pubmed CrossRef
  44. Zhang, YH, Heulsmann, A, Tondravi, MM, Mukherjee, A, and Abu-Amer, Y (2001). Tumor necrosis factor-alpha (TNF) stimulates RANKL-induced osteoclastogenesis via coupling of TNF type 1 receptor and RANK signaling pathways. J. Biol. Chem. 276, 563-568.
    Pubmed CrossRef
  45. Zhuang, S, Yan, Y, Daubert, RA, Han, J, and Schnellmann, RG (2007). ERK promotes hydrogen peroxide-induced apoptosis through caspase-3 activation and inhibition of Akt in renal epithelial cells. Am. J. Renal Physiol. 292, 440-447.
    Pubmed CrossRef


This Article


Cited By Articles
  • CrossRef (0)

Services
Social Network Service

e-submission

Archives