Biomol Ther  
Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors
Hyangmi Kim1, Nayoung Yi1, Byung-Rok Do2 and Ai-Young Lee1,*
1Department of Dermatology, Dongguk University Ilsan Hospital, Goyang 10326,
2Biotechnology Research Institute, Hurim BioCell Inc., Seoul 07531, Republic of Korea
E-mail: lay5604@naver.com
Tel: +82-31-961-7250, Fax: +82-31-961-7695
Received: October 23, 2018; Revised: November 15, 2018; Accepted: November 15, 2018; Published online: December 11, 2018.
© The Korean Society of Applied Pharmacology. All rights reserved.

Abstract
Coculture with adipose-derived stem cells (ADSCs) can stimulate proliferation and migration of melanocytes. To enhance outcomes of skin disorders caused by melanocyte loss or death, mixed transplantation with ADSCs has been suggested. However, role of cocultured ADSCs in proliferation and migration of melanocytes remains unclear. This study determined the effect of ADSCs on production of growth factors and expression levels of intergrins in primary culture of adult human melanocytes with or without ADSCs and in nude mice grafted with such melanocytes. Higher amounts of growth factors for melanocytes, such as bFGF and SCF were produced and released from ADSCs by coculturing with melanocytes. Relative levels of integrins β1, α5, and α6 as well as adhesion to fibronectin and laminin were increased in melanocytes cocultured with ADSCs. Such increases were inhibited by neutralization of bFGF or SCF. Relative levels of bFGF, SCF and integrins were increased in nude mice skin after grafting with melanocyte+ADSC cocultures. Collectively, these results indicate that ADSCs can stimulate proliferation and migration of melanocytes by increasing expression of integrins in melanocytes through upregulation of production/release of melanocyte growth factors such as bFGF and SCF.
Keywords: Adipose-derived stem cell coculturing, Melanocytes, Growth factors, Integrins


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