Biomol Ther 2018; 26(6): 584-590
Cytoprotective Effect of Taurine against Hydrogen Peroxide-Induced Oxidative Stress in UMR-106 Cells through the Wnt/β-Catenin Signaling Pathway
Jing Lou1,†, Donghe Han2,†, Huihui Yu1, Guang Yu1, Meihua Jin1,3,* and Sung-Jin Kim4,*
Departments of 1Immunology, 2Neurobiology, Jinzhou Medical University, Jinzhou 121001,
3Key Laboratory of Follicular Development and Reproductive Health of Liaoning, Jinzhou 121001, China
4Departments of Pharmacology and Toxicology, Metabolic Diseases Research Laboratory, School of Dentistry, KyungHee University, Seoul 02447, Republic of Korea
E-mail: (Jin M), (Kim SJ)
Tel: +86-416-4673251 (Jin M), +82-2-961-0868 (Kim SJ)
Fax: +86-416-4673162 (Jin M), +82-2-960-0867 (Kim SJ)
†The first two authors contributed equally to this work.
Received: March 19, 2018; Revised: May 31, 2018; Accepted: June 5, 2018; Published online: July 30, 2018.
© The Korean Society of Applied Pharmacology. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Osteoporosis development is closely associated with oxidative stress and reactive oxygen species (ROS). Taurine has potential antioxidant effects, but its role in osteoblasts is not clearly understood. The aim of this study was to determine the protective effects and mechanisms of actions of taurine on hydrogen peroxide (H2O2)-induced oxidative stress in osteoblast cells. UMR-106 cells were treated with taurine prior to H2O2 exposure. After treatment, cell viability, apoptosis, intracellular ROS production, malondialdehyde content, and alkaline phosphate (ALP) activity were measured. We also investigated the protein levels of β-catenin, ERK, CHOP and NF-E2-related factor 2 (Nrf2) along with the mRNA levels of Nrf2 downstream antioxidants. The results showed that pretreatment of taurine could reverse the inhibition of cell viability and suppress the induced apoptosis in a dose-dependent manner: taurine significantly reduced H2O2-induced oxidative damage and expression of CHOP, while it induced protein expression of Nrf2 and β-catenin and activated ERK phosphorylation. DKK1, a Wnt/β-catenin signaling inhibitor, significantly suppressed the taurine-induced Nrf2 signaling pathway and increased CHOP. Activation of ERK signaling mediated by taurine in the presence of H2O2 was significantly inhibited by DKK1. These data demonstrated that taurine protects osteoblast cells against oxidative damage via Wnt/β-catenin-mediated activation of the ERK signaling pathway.
Keywords: Taurine, Oxidative stress, Antioxidants, Wnt/β-catenin, Osteoblast

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Funding Information
  • National Natural Science Foundation of China(NSFC)
      81300534, 81300620
  • Natural Science Foundation of Liaoning Province(Liaoning Provincial Natural Science Foundation)
      2013022030, 2013022026

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