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Fig. 3. Amygdalin regulates apoptosis-related proteins. (A) Hs578T cells were treated with amygdalin at the indicated concentrations (0, 10, 20 and 40 mg/mL). The levels of Bax, Bcl-2, pro-caspase-3 and cleaved PARP were determined by immunoblot analysis using specific antibodies. (B) Hs578T cells were treated with 40 mg/mL amygdalin at the indicated time-points. The levels of phosphorylated and total p38 MAPK were determined by immunoblot analysis. (C) Hs578T cells were treated with 40 mg/mL amygdalin and 25 μM Z-VAD-FMK for 24 hr. The protein levels of cleaved PARP and pro-caspase-3 were determined by immunoblot analysis. (D) Hs578T cells were treated with 60 mg/mL amygdalin and 25 μM Z-VAD-FMK for 24 hr and then subjected to an MTT assay. The results represent mean ± SD for triplicates (*p<0.05 vs. control).
Biomol Ther (Seoul) 2016;24:62~66 https://doi.org/10.4062/biomolther.2015.172
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