Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

Download original image
Fig. 2. CaV3.1 mRNA and protein levels are increased in cultured cortical NPCs by VPA treatment. (A) mRNA levels of Cacna1g, 1h, and 1i were measured through quantitative real-time PCR in the NPCs 24 h after VPA treatment. The mRNA levels of T-type calcium channels were significantly increased by VPA treatment (N=4 per group, t-test, Cacna1g, t(6)=2.822, p=0.0303, Cacna1h, t(6)=3.196, p=0.0187, Cacna1i, t(6)=3.018, p=0.0235). (B) Western blots of CaV3.1, acetylated histone H3, histone H3, and β-actin and quantitative analysis of CaV3.1 protein levels. β-actin was used as the loading control for quantification of CaV3.1 on blots (N=6 per group, t-test, t(12)=4.88, p=0.0004). (C) Immunostaining of CaV3.1 in VPA-exposed NPCs at DIV1. Data are presented as mean ± SEM. N=4-6, *p<0.05 and ***p<0.001.
Biomolecules & Therapeutics 2020;28:389~396 https://doi.org/10.4062/biomolther.2020.027
© Biomolecules & Therapeutics