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Fig. 2. CaV3.1 mRNA and protein levels are increased in cultured cortical NPCs by VPA treatment. (A) mRNA levels of Cacna1g, 1h, and 1i were measured through quantitative real-time PCR in the NPCs 24 h after VPA treatment. The mRNA levels of T-type calcium channels were significantly increased by VPA treatment (N=4 per group, t-test, Cacna1g, t(6)=2.822, p=0.0303, Cacna1h, t(6)=3.196, p=0.0187, Cacna1i, t(6)=3.018, p=0.0235). (B) Western blots of CaV3.1, acetylated histone H3, histone H3, and β-actin and quantitative analysis of CaV3.1 protein levels. β-actin was used as the loading control for quantification of CaV3.1 on blots (N=6 per group, t-test, t(12)=4.88, p=0.0004). (C) Immunostaining of CaV3.1 in VPA-exposed NPCs at DIV1. Data are presented as mean ± SEM. N=4-6, *p<0.05 and ***p<0.001.
Biomolecules & Therapeutics 2020;28:389~396
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