Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 1. CaV3.1 mRNA and protein levels are increased in embryonic cortex exposed to VPA. Pregnant rats were exposed to VPA at E12.5, and T-type calcium channel mRNA (Cacna1g, Cacna1h, and Cacna1i) levels and CaV3.1 protein levels were analyzed in the embryo cortex on E14.5. (A) Quantitative real-time PCR results showing mRNA levels of T-type calcium channel subtypes. Cacna1g and Cacna1h mRNA levels are significantly increased by VPA treatment, and there was an increasing trend in the mRNA level of Cacna1i (Veh, n=5, VPA, n=6, t-test, Cacna1g, t(9)=2.441, p=0.0373, Cacna1h, t(9)=2.474, p=0.0354, Cacna1i, t(9)=1954, p=0.0824). (B) Blots of CaV3.1, acetylated histone H3, histone H3, and β-actin and quantitative analysis of CaV3.1 protein levels. β-actin was used as the loading control for quantification of the CaV3.1 on blots (N=7 per group, t-test, t(12)=4.88, p=0.0004). Data are presented as mean ± SEM. *p<0.05 and ***p<0.001.
Biomolecules & Therapeutics 2020;28:389~396
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