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Fig. 2. Time course and Na+-dependence of [3H]choline uptake in LNCaP cells. (A) Time course of [3H]choline uptake (10 μM) in the presence (●) and absence (○) of extracellular Na+ in LNCaP cells over 60 min. Na+-free buffer was modified by replacing NaCl with an equimolar concentration of NMDG-Cl. The time course of [3H]choline uptake was fitted to the experimental data using linear regression. (B) The kinetics of [3H]choline uptake in LNCaP cells. Cells were incubated for 20 min with 2.31 to 50 μM [3H]choline. Inset: Eadie-Hofstee plots of [3H]choline uptake show single straight lines. All data are presented as the mean ± SD (n=4).
Biomolecules & Therapeutics 2020;28:195~201
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