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Fig. 6. Knockdown of G0S2 activates the Hippo pathway via integrin-mediated FAK-Src. MDA-MB-231 cells were treated with G0S2 siRNA for 48 h. (A) Alteration of LATS1, YAP1, and CYR61 mRNA levels was identified using qPCR. All results were normalized to 18S rRNA. The data is shown as mean ± SEM (n=3). Statistically significant differences from control when *p<0.05. (B) Transfected cells were harvested, and total cellular proteins were extracted for western blot assay. The proteins were separated by SDS-PAGE (8%) and western blot assay was performed using indicated antibodies. The band intensities were measured with Quantity One software. Protein levels were normalized against α-tubulin levels. The data is shown as mean ± SEM (n=3). Statistically significant differences from control when *p<0.05. (C) Confocal microscopy was performed to observe alteration in expressions of YAP1 (left) and phosphorylated YAP1 (right) by G0S2 inhibition. Scale bar indicates 20 μm.
Biomolecules & Therapeutics 2019;27:591~602 https://doi.org/10.4062/biomolther.2019.063
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