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Fig. 4. Alteration of skin differentiation in 9-week old Rab25 KO mice. (A) Images of western blots for representative keratinocyte differentiation markers using skin specimens from WT and Rab25 KO mice. GAPDH was used for housekeeping control. (B) Images of western blots for representative keratinocyte differentiation marker. Knockdown-HaCaT cell (sh1, sh2, and sh3) line was constructed by infection with the recombinant pGIPZ vector, using polybrene mixture. Expression level of the knockdown cell was compared to that of non-infected cell (WT) and vector-conveying cell (shCon). GAPDH was used for housekeeping control. (C) Schematic image of cluster analysis-hierarchical clustering heat map acquired from Affymetrix GeneChip® Mouse Gene 2.0 ST Array (Thermo) using skin specimen of WT and Rab25 KO mice. The heat map was represented and sorted by the normalized value of expression level using each probe. (D) Bar graph indicated top 10 biological processes with the highest p-value. GO functional analysis was based on gomap_stat. (E) mRNA expression was normalized with GAPDH. Graphs represent mean ± SEM (Unpaired student’s t-test, n=4, *p<0.05, **p<0.01, ***p<0.001).
Biomolecules & Therapeutics 2019;27:553~561 https://doi.org/10.4062/biomolther.2019.125
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