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Fig. 2. Functional analysis of helix 8 chimeras of D1R and D2R. (A) Subcellular localization of WT and helix 8 chimeras of D1R and D2R was visualized by immunofluorescence using confocal microscopy. Upper panels show non-permeabilized cells, and lower panels show permeabilized cells with blue presenting nucleus (DAPI staining) and red presenting receptors. Scale bars represent 10 μm. (B) Standard curve of cAMP generation according to surface-expression of WT D1R. Surface-expression level is plotted on the x-axis, and the y-axis is a percentage of maximal cAMP accumulation induced by 100 nM dopamine treatment relative to 10 μM forskolin (Fsk). (C) Effect of chimeric mutation on cAMP accumulation induced by 100 nM dopamine. Error bars represent STDEV.
Biomolecules & Therapeutics 2019;27:514~521
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