The PCR primer sequences use in real-time quantitative PCR
| Target gene | Forward (5'-3') | Reverse (5'-3') |
|---|---|---|
| TRAP | ACACAGTGATGCTGTGTGGCAACTC | CCAGAGGCTTCCACATATATGATGG |
| NFATc1 | GGGTCAGTGTGACCGAAGAT | GGAAGTCAGAAGTGGGTGGA |
| c-Fos | CCAGTCAAGAGCATCAGCAA | AAGTAGTGCAGCCCGGAGTA |
| MMP9 | AGTTTGGTGTCGCGGAGCAC | TACATGAGCGCTTCCGGCAC |
| Cathepsin K | AGGCGGCTATATGACCACTG | CCGAGCCAAGAGAGCATATC |
| β-actin | TCACCCACACTCTGCCCAT | TCCTTAATGTCACGCACCATTT |
TRAP, Tartrate-resistant acid phosphatase; NFATc1, Nuclear factor of activated T cells; MMP9, Matrix Metallopeptidase 9.