Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

Download original image
Fig. 4. FX-9 influences JAK/STAT, MAPK and PI3K/AKT pathway. SEM and RS4;11 cells were exposed to DMSO, 2.5 μM or 5.0 μM FX-9 for 48 h. For quantification, Image Studio Lite software (Version 5.2, LI-COR Biotechnology) was used to determine signal intensities of the protein bands. Protein bands were normalized to the GAPDH band of the respective sample and the ratio of phosphorylated forms to the corresponding total protein signal intensities was calculated. Values are expressed as ratio. DMSO control is considered as value 1 and samples were measured in relation to the control. (A) FX-9 decreased levels of p-STAT3 in SEM cells and increased pERK protein level in RS4;11 (B) FX-9 reduced levels of p-AKT and p-p38 in SEM cells. Exemplary results for SEM and RS4;11 cells are displayed. GAPDH was used as loading control.
Biomol Ther 2019;27:492~501 https://doi.org/10.4062/biomolther.2018.199
© Biomolecules & Therapeutics