Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 5. Autophagy by serum starvation inhibited curcumin-induced cell death. (A) A172 glioblastoma cells were incubated under serum starvation for 3, 6, 12, 24 h. Cell lysates were prepared and LC3 proteins were detected by western blotting. (B) A172 cells were incubated under serum starvation for 6 h. Autophagic cells with LC3 puncta were counted under fluorescence microscope with 1,000X magnification (B). (C) A172 cells were incubated under serum starvation for 24 and 48 h. Then, cell viability was measured by MTT assay as described in materials and methods. (D) A172 cells were incubated under serum starvation for 12 h. DNA condensation was assessed by DAPI staining. (E, F) A172 cells were treated with 10 μM curcumin in the presence or absence of 10% serum for 1, 3, 6 and 9 h. Cell lysates were prepared and LC3 proteins were detected by western blotting (E). DNA condensation at 6 h was assessed by DAPI staining (F). Data in bar graph represent mean ± SED. &&p<0.01, significantly different from control in the presence of 10% serum (B–D, F). **p<0.01, significantly different from curcumin-untreated each control in the presence or absence of 10% serum (F). ##p<0.01, significantly different from curcumin-treated in the presence of 10% serum (F).
Biomol Ther 2019;27:484~491 https://doi.org/10.4062/biomolther.2019.107
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