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Fig. 3. Cell death was increased by the treatment with rapamycin, autophagy inducer. (A–C) A172 glioblastoma cells were treated with 100 nM rapamycin for 1, 3, and 6 h. Cell lysates were prepared and LC3 proteins were detected by western blotting (A). Autophagic cells with LC3 puncta were counted under fluorescence microscope with 1,000X magnification (B). Cell viability was measured by MTT assay as described in materials and methods (C). (D–F) A172 cells were treated with 100 nM rapamycin in the presence of autophagy inhibitors such as HCQ (D), 3-MA (E) or LY294002 (F) for 24 h. Then, cell viability was measured by MTT assay as described in materials and methods. Data in bar graph represent mean ± SED. *p<0.05; **p<0.01, significantly different from rapamycin-untreated and each autophagy inhibitor-untreated control. #p<0.05; ##p<0.01, significantly different from rapamycin-treated and each autophagy inhibitor-untreated control.
Biomol Ther 2019;27:484~491 https://doi.org/10.4062/biomolther.2019.107
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