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Fig. 2. Autophagy inhibitors attenuated curcumin-induced cell death. (A–C) A172 glioblastoma cells were treated with 10 μM curcumin in the presence of autophagy inhibitors such as hydroxyquinoline (HCQ) (A), 3-methyladenine (3-MA) (B) or LY294002 (C) for 3 h. Then, cell lysates were prepared and LC3 proteins were detected by western blotting. (D–I) A172 cells were treated with 10 μM curcumin in the presence of autophagy inhibitors such as HCQ (D, G), 3-MA (E, H) or LY294002 (F, I) for 24 h. Then, cell viability was measured by MTT assay as described in materials and methods (D–F). Dead cells were estimated by trypan blue exclusion assay (G–I). Data in bar graph represent mean ± SED. *p<0.05; **p<0.01, significantly different from curcumin-untreated and each autophagy inhibitor-untreated control. #p<0.05; ##p<0.01, significantly different from curcumin-treated and each autophagy inhibitor-untreated control.
Biomol Ther 2019;27:484~491 https://doi.org/10.4062/biomolther.2019.107
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