Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 1. Curcumin treatment enhanced cell death and autophagy in glioblastoma cells. (A–C) A172 glioblastoma cells were treated with 10 μM curcumin for 12, 24, and 48 h. Then, cell viability was measured by MTT assay as described in materials and methods (A). Dead cells were estimated by trypan blue exclusion assay (B). DNA condensation was assessed by DAPI staining (C). (D) A172 cells were treated with 10 or 20 μM curcumin for 24 h and dead cells were estimated by trypan blue exclusion assay. (E–G) A172 cells were treated with 10 μM curcumin for 1, 2 and 3 h. Cell lysates were prepared and LC3 proteins were detected by western blotting (E). LC3 puncta formation was observed under fluorescence microscope with 1,000X magnification. Arrows indicated representative LC3 puncta (F). Autophagic cells with LC3 puncta were counted (G). Data in bar graph represent mean ± SED. **p<0.01, significantly different from curcumin-untreated control (A–D, G).
Biomol Ther 2019;27:484~491 https://doi.org/10.4062/biomolther.2019.107
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