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Fig. 4. RLYE inhibits VEGF-A-induced neovascularization and vascular permeability in mice. (A, B) Mice were subcutaneously injected with 0.5 mL of Matrigel containing VEGF-A (100 ng) alone or in combination with RLYE (0.75 pmol). (A) After 7 days, Matrigel plugs were excised from the mice after sacrifice by cervical dislocation and photographed. (B) Hemoglobin (Hb) concentrations were measured after extraction from the Matrigel plugs using the Drabkin method (n=4). (C, D) Mice were injected intraperitoneally with RLYE (1 mg/100 μL/kg). After 4 h, the mice were injected intravenously with 1% Evans blue following anesthesia, followed by intradermal injection of PBS (50 μL/site) or VEGF-A (100 ng/50 μL/site). (C) The mice were euthanized after 20 min, and the dorsal skin was carefully removed and photographed. (D) Evans blue dye was extracted from the skin with formamide, and the dye concentration was determined by spectrophotometry (n=4). **p<0.01.
Biomol Ther 2019;27:474~483
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