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Fig. 2. DA suppresses Th17 differentiation and function. CD4+ T cells were activated and cultured in the presence of DA under Th17 polarizing condition for 5 days. (A) Cells were analyzed by intracellular cytokine staining. Shown are representative FACS profiles in the live CD4 T cell gate: inset numbers indicate the mean (± SD) percentages of IL-17+ cells obtained from three independent experiments. (B) Cells were restimulated with anti-CD3e and anti-CD28 for 24 h, and the culture supernatants were analyzed by CBA. (C, D) Th17 cells were cultured as in (A) and analyzed by quantitative RT-PCR. Concentrations of the indicated mRNAs were normalized to those of Actb, and the means (± SD) of the relative levels from three biological replicates are shown: *p<0.05, **p<0.01.
Biomol Ther 2019;27:466~473
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