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Fig. 2. CYC116 reversibly suppresses degranulation in antigen-stimulated Mast cells. (A) The amount of β-hexosaminidase released from BMMCs was determined as described in “Materials and Methods”. (B) BMMCs were washed 3 times after pre-incubation with or without CYC116 (5 µM) or PP2 for 30 min. The cells were stimulated with 50 ng/ml antigen for 15 min. (C) BMMCs were incubated with or without CYC116 for 4 h and cytotoxicity was examined by CCK-8 as described in “Materials and Methods”. The values are presented as the means ± SEM from three independent experiments in triplicate. Significant differences compared to antigen-only group are indicated (A) or as indicated (B), **p<0.01. Ag, antigen; PP2, a typical Src-family kinase inhibitor.
Biomolecules & Therapeutics 2019;27:311~317
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