Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 2. Justicidin A decreased secreted Aβ level. (A) APPsw-transfected HeLa cells were incubated with indicated concentrations of justicidin A for 8 h. The levels of Aβ in the medium were measured using ELISA methods. % of control is obtained by normalizing to the control for each experimental condition. Justicidin A decreased the levels of Aβ42 (open bars, n=6) and Aβ40 (closed bar, n=6). (B) APPwt-transfected SH-SY5Y cells were incubated with indicated concentrations of justicidin A for 4 h, and the Aβ42 level was measured from the conditioned media using ELISA methods. Justicidin A decreased secreted Aβ42 level (n=4). (C, D) APPsw-transfected HeLa cells were incubated with indicated concentrations of justicidin A for 8 h. The levels of sAPPβ-sw, and sAPPα in the medium were measured using ELISA methods. sAPPβ-sw level (C) was decreased and sAPPα level (D) was increased by justicidin A (n=5). (E) Levels of sAPPα and sAPPβ in the medium were measured using Western blot. (F) Bars indicate the levels of sAPPα and sAPPβ obtained from densitometric analysis of Western bands in (E) (n=4). *p<0.05, **p<0.01, ***p<0.001.
Biomol Ther 2019;27:276~282 https://doi.org/10.4062/biomolther.2018.112
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