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Fig. 2. Evaluation of enrichment of MLL1-binding aptamers during SELEX. (A) Binding activity was measured using various amounts of S0, S3, and S16 libraries and 1 μg of MLL1 protein. The binding activity was calculated by qRT-PCR as described in “Materials and Methods”. Data are presented as means ± SEM. (B) Sequencing data of S0, S3, and S16 libraries were obtained using NGS. The percentage population of the five most popular abundant aptamers was revealed. (C) Sequence abundance in the sub-population consisting of the top 50 sequences. The percentage in the population of top 50 unique sequences was determined from S1, S3, and S16 libraries.
Biomolecules & Therapeutics 2019;27:201~209
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