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Fig. 3. Effects of EC on METH-induced ROS accumulation and reduction of MMP in HT22 hippocampal neuronal cells. (A) Cells were pretreated with EC for 1 h, and incubated with 1 mM METH for 24 h. Cells were then incubated with 5 μM DCF-DA for 15 min. The intracellular ROS level was determined using a fluorescence microscope. (B) Cells were pretreated with EC for 1 h, and treated with 5 mM METH for 24 h. Cells were stained with TMRM and the fluorescence was measured by flow cytometry (left). Percentage of MMP was calculated and plotted in the graph (right). *p<0.01 when compared with untreated control cells. #p<0.01 when compared with METH-treated cells.
Biomol Ther 2019;27:145~151 https://doi.org/10.4062/biomolther.2018.092
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